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Table 1 Summary of downregulated proteins in A549 cells infected with influenza A H5N1 virus compared to H9N2-infected cells at 24 hpi (r > 2, p < 0.05)

From: Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9

Spot no.a Accession No.b Gene Protein name MW (Da) pI Scorec
1 P04264 KRT1 Keratin, type II cytoskeletal 1 66,170 8.15 120
2 P10809 HSPD1 60-kDa heat shock protein, mitochondrial 61,187 5.7 69
3 B4DEF7 N /A cDNA FLJ60062, highly similar to 78-kDa glucose-regulated protein 30,458 5.77 68
4 Q59FC6 N /A Tumor rejection antigen (Gp96) 1 variant 66,140 5.08 46
5 V9HWE1 HEL113 Epididymis luminal protein 113 53,676 5.24 156
6 Q7L4M3 KRT 8 KRT8 protein 30,802 5.05 57
7 P07237 P4HB Protein disulfide-isomerase 57,146 5.96 60
8* P06576 ATP5F1B ATP synthase subunit beta, mitochondrial 56,525 5.26 130
9 Q9UJZ1 STOML2 Stomatin-like protein 2, mitochondrial 38,839 4.45 120
10 P35908 KRT2 Keratin, type II cytoskeletal 2 epidermal 65,678 8.07 47
11 P13645 KRT10 Keratin, type I cytoskeletal 10 58,994 5.13 49
12 A8K401 PHB Prohibitin, isoform CRA_a 29,843 4.14 175
13 P30044 PRDX5 Peroxiredoxin-5, mitochondrial 17,611 9.02 50
14 Q9BYX7 POTEKP Putative beta-actin-like protein 3 42,331 5.91 53
15* Q6FHZ0 MDH2 Malate dehydrogenase, mitochondrial 35,965 8.92 63
16 P38646 HSPA9 Stress-70 protein, mitochondrial 73,967 6.03 52
  1. aSpot No. is the unique sample spot protein number
  2. bAccession No is the MASCOT result of MALDI-TOF/TOF searched from the NCBI database
  3. cProtein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification
  4. *Indicating that the differential proteins were validated by western blot analysis
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Virology Journal

ISSN: 1743-422X